SOB, 454g powder, (31g/Litre)
Contents/Litre: 20g tryptone-B, 5g yeast extract, 0.5g NaCl, 5g MgSO4.7H2O.
For preparing competent host cells prior to transformation, cells are grown in SOB.
I used KAPA HiFi HS to amplify a 7kb component of the nuclear pore complex, which I had difficulties getting a clean amplification. The result was very satisfying and superior to all the other high-fidelity, proof-leading polymerases used in our lab.
Dr P Louphrasitthiphol, Oxford University, 29 January 2013
The Kapa Illumina library quantification kit is easy to use and allows all types of Illumina libraries to be quantified quickly.
The kit contains a primer premix that is directly added to the 2x Kapa SYBR Fast qPCR Master Mix before use. Having the master mix already prepared helps to dramatically reduce the time spent setting up each qPCR plate. The kit also contain 6 DNA standards which allow quantification of library dilutions up to a concentration of 20 pM.
This kit has greatly helped us to create reproducible cluster numbers on each Illumina NGS run and platform used and is a real necessity for anyone quantifying Illumina libraries.
Dr S Holden, Manchester University, 20 November 2012