Now You Can Reliably Amplify Your Long Templates - up to 20 kb
The KAPA Long Range PCR system is a blend of Taq DNA polymerase and an engineered archaeal (Type B) DNA polymerase possessing proofreading capability. This two-enzyme system is designed specifically to support long range and/or sensitive PCR from complex targets. It possess three to four times higher fidelity than Taq polymerase.
The KAPA Long Range PCR system is ideally suited for PCR amplification of long targets up to 20 kb and/or PCR using low amounts of template DNA. It will also improve results in standard short- and mid-range PCR amplification, production of PCR products to be used for ligation into 3’-T-overhang cloning vectors and targeted sample enrichment for next-generation DNA sequencing.
KAPA Long Range DNA Polymerase is also available with HotStart technology for improved specificity during prolonged benchtop setup or liquid handling. In the hot start formulation, the enzyme is combined with a proprietary antibody that inactivates the enzyme until the first denaturation step. This eliminates spurious amplification products resulting from non-specific priming events during reaction setup and initiation, and increases overall reaction efficiency.
17 September 2009