Get Outstanding PCR Performance and Reduce Cycling Time by Up to 70% with New Bioengineered KAPA2G Fast Hot Start DNA Polymerase

Developed using Kapa Biosystems’ molecular evolution technology this enzyme provides significantly faster processivity than wild-type Taq polymerase. KAPA2G Fast Hot Start provides extension rates as low as one second per kilobase reducing total reaction time by 20 - 70%. It generates higher yields and sensitivity than other enzymes across a wide range of amplicon types but requires no specialist PCR consumables or instrumentation.

In the Hot Start formulation, the enzyme is combined with a proprietary antibody that inactivates the enzyme until the first denaturation step. This eliminates spurious amplification products resulting from non-specific priming events during reaction setup and initiation, and increases overall reaction efficiency. With KAPA2G Fast Hot Start there is no need for lengthy re-optimisation of your reactions. Any standard, end-point PCR assay performed efficiently with wild-type Taq polymerase (or a hot start formulation thereof) can be easily converted to a Fast PCR assay with KAPA2G Fast Hot Start. Amplicons generated with KAPA2G Fast Hot Start are suitable for routine downstream applications, including restriction enzyme digestion, cloning and sequencing.
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To request your FREE sample, contact us now on + 44 (0) 1582 747500 or e-mail response@anachem.co.uk (UK & Ireland customers only).

Download the technical brochures for the complete Kapa range