Eliminate the need for DNA purification with KAPA Blood PCR kits, containing the first DNA polymerase evolved for whole blood PCR.
Reduce contamination risk, turnaround time and cost of genetic testing
High-throughput genetic testing directly from blood has not been feasible with wild-type polymerases, due to the presence of multiple PCR inhibitors in whole blood. KAPA Blood DNA Polymerase was evolved in this environment and offers the ability to amplify DNA fragments directly from blood, without any pretreatment of blood samples or DNA isolation. This not only reduces the cost and turnaround time of genetic testing, but also significantly reduces the risk of sample cross-contamination associated with the need for DNA extraction.
- Direct amplification from fresh or frozen whole blood, blood collected in EDTA tubes or on FTA® Elute Cards, “Guthrie cards” or filter paper.
- Eliminates the need for DNA extraction in genetic testing protocols for humans, other mammals and birds.
- Reduces contamination risk, turnaround time and cost of genetic testing.
- Compatible with existing workflows, standard protocols and routinely used detection methods.
Amplification of a 459 bp fragment of exon 19 of the human Duchenne muscular dystrophy gene directly from whole human blood, using KAPA Blood PCR Mix B.
Product Description
KAPA Blood PCR Kits contain KAPA Blood DNA Polymerase, a second-generation enzyme derived through a process of molecular evolution and the first DNA polymerase engineered specifically for the amplification of DNA directly from whole blood.
The enzyme is available in two optimized, easy-to-use 2x PCR mixes, which contain all components required for Whole Blood PCR, except primers and template (blood). Using KAPA Blood PCR Kits, DNA fragments may be amplified directly from reactions containing 1 - 20% (v/v) whole human blood without pretreatment of blood samples or DNA isolation, significantly reducing contamination risk, turnaround time and cost of genetic testing.
KAPA Blood PCR Mix A is preferred for assays employing highly sensitive fluorescent detection systems. KAPA Blood Mix B is formulated for higher yields and GC-rich amplicons and is recommended when analysis is by agarose gel electrophoresis and ethidium bromide staining. Products generated with KAPA Blood PCR Mixes A or B may be analyzed by restriction endonuclease digestion, DNA sequencing or dHPLC analysis.
Product Applications
KAPA Blood PCR Kits have been validated for the direct amplification of DNA fragments from fresh or frozen whole blood, blood collected in EDTA anti-coagulant tubes, on FTA® Elute Cards, Whatman 903® Specimen Collection Paper (“Guthrie cards”) or regular filter paper. The optimal amount of blood per reaction depends on the species, amplicon type and application. KAPA Blood PCR Kits are recommended for the following:
- Genetic testing of humans, other mammals and birds.
- Amplicons with a GC content <65% up to 3.5 kb in length, and amplicons with GC content >65% up to 1 kb.
- Single amplicon or Multiplex PCR assays, using unlabelled or fluorescently labelled primers.
- Routinely used detection methods including restriction endonuclease digestion, agarose gel electrophoresis/ethidium bromide staining, fluorescent capillary electrophoresis, DNA sequencing and dHPLC analysis.
- KAPA Blood PCR Kits are ideally suited for paternity testing using the PowerPlex® 16 System (Promega).
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