Next Generation PCR Enzymes from Kapa Biosystems

 

Despite the various claims of other reagents suppliers, all commercially available Taq polymerase is the same wild-type enzyme – any differences in performance are due to buffer formulation and/or enzyme concentration.

Kapa Biosystems’ next-gen PCR reagents, now available from Anachem, are fundamentally different at the protein level. These engineered polymerases developed through high-throughput molecular evolution technology, contain unique amino acid modifications that confer dramatic improvements to the function of the enzyme.

This platform is being used to develop a portfolio of designer polymerases engineered specifically for applications such as qPCR, Fast PCR, high fidelity PCR, crude sample PCR, next-generation DNA sequencing, and molecular diagnostics.

>>Choose the best Kapa Enzyme for Your Application
>>See a YouTube news report on Kapa Biosystems 

The range of Kapa enzymes includes:

KAPAHiFi HotStart DNA Polymerase
KAPA Blood PCR Kits
KAPA3G Plant PCR Kits
A second-generation polymerase engineered for extreme fidelity and robustness. KAPAHiFi HotStart DNA Polymerase is a novel, single-enzyme system that exhibits industry-leading performance when compared with other high fidelity polymerases and polymerase blends. Eliminate the need for DNA purification with the first polymerase engineered for PCR direct from blood. KAPA Blood PCR Kits are available in two optimized, easy-to-use 2x formats containing all PCR components except primers and template (whole blood) Based on a novel, third-generation (3G) DNA polymerase, engineered via molecular evolution for improved tolerance to common plant-derived PCR inhibitors such as polyphenolics and polysaccharides. Fast PCR direct from plant tissues such as leaf discs, seeds and crude plant extracts.

KAPA SYBR FAST qPCR Kits

KAPA2G Robust HotStart

 


KAPA Mouse Genotyping Kits
KAPA SYBR® FAST qPCR Kits are designed for the highest performance in real-time PCR. The kit is based on a novel DNA polymerase engineered through a process of molecular evolution. The result is a unique enzyme, specifically evolved for qPCR using SYBR® Green I dye chemistry. KAPA2G Robust HotStart is a highly robust and versatile second-generation enzyme derived through a process of molecular evolution. The novel amino acid mutations in the flagship KAPA2G Robust DNA Polymerase offer higher processivity and specific activity, which translates to robust performance.

Rapid extraction and amplification of DNA from mouse tissue in less than 1 hour.
 

KAPA EXpress Extract - 15 minute extraction using no organic solvents and requiring no pH neutralization or centrifugation.
KAPA2G Fast Genotyping Mix - 45 minute amplification using a novel DNA polymerase engineered for high processivity and extreme speed.


KAPAHiFi DNA Polymerase

KAPA2G Fast HotStart DNA Polymerase

KAPA Long Range PCR Kits
 
A second-generation polymerase engineered for extreme fidelity and robustness. KAPAHiFi DNA Polymerase is a novel, single-enzyme system that exhibits industry-leading performance when compared with other high fidelity polymerases and polymerase blends. KAPA2G Fast HotStart is a second-generation enzyme derived through a process of molecular evolution. KAPA2G Fast DNA Polymerase was specifically engineered for higher processivity and speed, offering significantly faster extension rates than wild-type Taq polymerase. A blend of Taq DNA polymerase and an engineered archaeal (B-family) DNA polymerase possessing proofreading capability. This two-enzyme system is designed specifically to support long range and/or sensitive PCR.
 

KAPA2G Robust DNA Polymerase

KAPA2G Fast DNA Polymerase

 


KAPA Library Kits
A second generation DNA polymerase engineered for extreme robustness and difficult templates. KAPA2G Robust DNA Polymerase is a highly versatile engineered enzyme that was derived from Kapa Biosystems’ molecular evolution platform. A second generation DNA polymerase engineered for extreme speed - also available in hot start for superior specificity and high-throughput PCR. Designed specifically for speed and high performance. Innovative solutions for next-generation sequencing library preparation, amplification and quantificiation.
 
 >>See Product Listings and Ordering Information

30 March 2009
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