Electroblotting Complete Unit for 4 10x10cm
TV100-EBK
Electroblotting Complete Unit for 4 10x10cm
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£ 638.40
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I used KAPA HiFi HS to amplify a 7kb component of the nuclear pore complex, which I had difficulties getting a clean amplification. The result was very satisfying and superior to all the other high-fidelity, proof-leading polymerases used in our lab.

 


Dr P Louphrasitthiphol, Oxford University, 29 January 2013


The Kapa Illumina library quantification kit is easy to use and allows all types of Illumina libraries to be quantified quickly.

The kit contains a primer premix that is directly added to the 2x Kapa SYBR Fast qPCR Master Mix before use. Having the master mix already prepared helps to dramatically reduce the time spent setting up each qPCR plate. The kit also contain 6 DNA standards which allow quantification of library dilutions up to a concentration of 20 pM.

This kit has greatly helped us to create reproducible cluster numbers on each Illumina NGS run and platform used and is a real necessity for anyone quantifying Illumina libraries.
 


Dr S Holden, Manchester University, 20 November 2012




Electroblotting Complete Unit for 4 10x10cm

The electroblotters provide a cost-effective and safe and easy to use system for the transfer of proteins and nucleic acids from mini and maxi gels. They can accommodate up to 4 electroblotting cassettes. The cassettes are asymmetric colour-coded a unique design feature to prevent them from being inserted into the tank in the wrong orientation, against the direction of transfer.

Benefits Include:
Asymmetric, colour-coded cassettes – hinged, with red and black covers corresponding to the anode and the cathode, to ensure correct transfer.
Fibre pads – compress the gel against the nitrocellulose membrane for uniform transfer.
Plate-electrodes – 12.8cm apart – maximise the intensity of the electric field, allowing rapid transfer within 4 to 6 hours/
Enhanced cooling – included as standard – snap lock connectors allow the cooling coil within each unit to be connected to the chiller unit for faster, high-intensity transfers or extended overnight runs without buffer depletion.
Flat bottom – allows each electroblotter to be placed directly onto a magnetic stirring plate.
Technical Specification
Unit Dimensions20 x 22.5 x 18.5cm
Inner Tank Dimensions12.8 x 11 x 17cm
Maximum Sample Capacity 4 cassettes
Recommended Buffer Volume2000ml
Recommended Running Conditions Voltage50-100V
for Electroblotting Current 75mA
Time 2-4h
Distance Between Plate Electrodes 12.8cm
In EBGRM
Electroblotting Cassette Dimensions 11.5 x 10.5cm
Active Transfer Area 71cm
Power OutputShrouded, 4mm
Connectors (diameter)
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