KAPA2G Robust HS ReadyMix 500 rxns
KK5702
KAPA2G Robust HS ReadyMix 500 rxns
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I used KAPA HiFi HS to amplify a 7kb component of the nuclear pore complex, which I had difficulties getting a clean amplification. The result was very satisfying and superior to all the other high-fidelity, proof-leading polymerases used in our lab.

 


Dr P Louphrasitthiphol, Oxford University, 29 January 2013


The Kapa Illumina library quantification kit is easy to use and allows all types of Illumina libraries to be quantified quickly.

The kit contains a primer premix that is directly added to the 2x Kapa SYBR Fast qPCR Master Mix before use. Having the master mix already prepared helps to dramatically reduce the time spent setting up each qPCR plate. The kit also contain 6 DNA standards which allow quantification of library dilutions up to a concentration of 20 pM.

This kit has greatly helped us to create reproducible cluster numbers on each Illumina NGS run and platform used and is a real necessity for anyone quantifying Illumina libraries.
 


Dr S Holden, Manchester University, 20 November 2012




KAPA2G Robust HS ReadyMix 500 x 25uL reactions (1 x 6.25mL)

KAPA2G Robust PCR Kits


Enhance your PCR workflows by consolidating protocols and reaction conditions, boost pass rates, amplify difficult templates without bias, and improve sensitivity – all with a single enzyme.


The second-generation KAPA2G Robust DNA Polymerase was evolved to solve - achieve steady amplification across a broad range of amplicon types (both GC and AT-rich). The versatility and robustness of the polymerase allows for consolidation of PCR cycling protocols and reaction conditions while increasing success rates. The high performance of the KAPA2G Robust DNA Polymerase eliminates the need for multiple enzymes and protocols - standardize your PCRs with a single enzyme solution.


The KAPA2G Robust DNA Polymerase offers:


  • Robust performance across a wide range of GC- and AT-rich templates
  • Increased PCR success rates
  • Consolidated PCR protocols and reaction conditions with a single enzyme
  • Improved tolerance to common PCR inhibitors for crude samples and/or DNA extractions
  • Consistent Colony PCR from E. coli and yeast
  • Higher yield and sensitivity per unit of enzyme
  • Three novel buffers and a proprietary PCR enhancer offer extended optimization options for the most difficult templates
  • KAPA2G Robust DNA Polymerase is also accessible with HotStart technology for improved specificity during prolonged benchtop setup or liquid handling.

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