KAPA2GFast HS PCR Mix with Dye 100rxn
KK5608
KAPA2GFast HS PCR Mix with Dye 100rxn
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I used KAPA HiFi HS to amplify a 7kb component of the nuclear pore complex, which I had difficulties getting a clean amplification. The result was very satisfying and superior to all the other high-fidelity, proof-leading polymerases used in our lab.

 


Dr P Louphrasitthiphol, Oxford University, 29 January 2013


The Kapa Illumina library quantification kit is easy to use and allows all types of Illumina libraries to be quantified quickly.

The kit contains a primer premix that is directly added to the 2x Kapa SYBR Fast qPCR Master Mix before use. Having the master mix already prepared helps to dramatically reduce the time spent setting up each qPCR plate. The kit also contain 6 DNA standards which allow quantification of library dilutions up to a concentration of 20 pM.

This kit has greatly helped us to create reproducible cluster numbers on each Illumina NGS run and platform used and is a real necessity for anyone quantifying Illumina libraries.
 


Dr S Holden, Manchester University, 20 November 2012




Kapa2G Fast HotStart PCR Master Mix with loading dye 100 reactions (1x1.25mL)

KAPA2G Fast PCR Kits

A second-generation DNA polymerase revived for greater speed and performance.
KAPA2G Fast DNA Polymerase is a second-generation enzyme imitated through a process of molecular evolution. The KAPA2G Fast DNA Polymerase was engineered for higher speed, offering considerably faster extension rates and improved PCR success rates than wild-type Taq polymerase. KAPA2G Fast is well suited for both routine and high-throughput PCR, achieving higher yields and improved sensitivity than competitor enzymes across a wide range of amplicon types.


KAPA2G Fast DNA Polymerase and optimised buffer system offers:


  • Extension times as low as 1 sec per kilobase.
  • Outstanding performance when compared to standard and hot start Taq polymerase.
  • 20 - 70% reductions in total PCR time.
  • No requirement for specialist PCR consumables or instrumentation.
  • KAPA2G Fast DNA Polymerase is also available with HotStart technology for improved specificity during prolonged benchtop setup or liquid handling.

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