Anachem Ltd. is THE pipette & laboratory supplies specialist in the UK & Ireland. Founded in 1970, our 40 years of exclusive distribution ensures we have the best expertise in pipetting technical support, maintenance & calibration, customer service & e-commerce. We are the people you can trust and rely on for all pipettes, pipettes tips, pipette calibration, general laboratory & PCR plastics, plus innovative new molecular biology reagents. Anachem is now a METTLER TOLEDO company.
Anachem's 40 years of expertise in pipetting makes it THE supplier of choice for an extensive range of the highest quality pipetting products from leading manufacturers such as RAININ. For all types of liquid handling tools: micropipette, volumetric pipette, automatic pipette, multichannel pipette, electronic pipette, bottle top dispenser, 96-well dispenser- they are all here.
The highest quality pipette tips including the Rainin BioClean line ensures you get maximum performance and no interference in your assays. Anachem provides innovative packaging systems for disposable tips that minimize environmental impact. Our tips fit a wide range of pipettes - get help finding the tips you need with our Pipette Tip Selector.
Sample Storage: our extensive range of high quality tubes, storage racks and labelling materials ensure your precious samples are protected, maintain their integrity and are easy to locate. From graduated 0.5ml microtubes and vials up to 15ml and 50ml centrifuge tubes, they are all made from the purest polypropylene.
96 well, 384 well and deep well microplates for your microtiter and high throughput applications. For PCR - diagnostic, quatitative or real time our PCR plates and PCR tubes are made from the purest materials to protect your samples and eliminate assay interference. Innovative features enhance sensitivity and detection levels of dna amplification. New 4titude plates offer superior performance at great value.
Protect yourself, your lab equipment and your samples against the safety hazards exposed to in your laboratory work. Anachem laboratory supplies provides effective decontamination solutions such as MicroSol3plus & Virkon for general disinfection and any microbiology lab. Protective gloves, trays and radiation safety shields also support your laboratory procedures in biological or chemical areas.
Key reagents and instrumentation for nucleic acid manipulations, DNA extraction, PCR & electrophoresis. New KAPA Biosystems Second Generation PCR polymerases bring advanced performance and yield, with specialist enzymes for real time PCR with SYBR® Green, Hot Start PCR etc.
The Rainin Liquidator®96 Manual 96-well Pipettor provides easy, simultaneous pipetting into 96 wells with accuracy and efficiency. Our other bench top instruments include peristaltic pumps, dilutor dispensers, mini centrifuge, stirrer and Safe Aspiration Station.
With precise results, easy cleaning and a design made to last, METTLER TOLEDO NewClassic balances help you deal with many scientific challenges, from boosting productivity, achieving high quality, to serving heightened customer demands. Swiss technology, precision and quality assist you in creating lasting value for your laboratory.
Whether you need solutions for complex applications under regulatory control or for simple routine measurements, METTLER TOLEDO’s comprehensive pH and conductivity range covers varied applications across all sectors. Backed by 60 years of expertise in sensor innovation and an extensive range of accessories, you can be assured of a product range of unparalleled quality and flexibility.
Anachem is the largest and most experienced pipette calibration & service organisation in the UK and Ireland. With over 30 years of expertise in all aspects of pipette maintenance for all makes and models there is no better support to ensure your pipette accuracy and precision. We have held UKAS accreditation for pipette calibration since 1998.
I had been trying to amplify a GC rich region of genomic DNA with poor quality samples. I tested several enzymes including Amplitaq 360, Thermo-Start, Herculase II and HotStar, but without much success. I was very impressed with the results of the Kapa Biosystems Robust PCR Kit, as it has given me much better amplification than any other enzyme and combination of buffers I have tried.
04 August 2009
I tried out the Kapa2G robust kit. We're super happy with it. We first tested it on gDNA and the amount of template needed was very minimal, 2ng in a 25ul reaction, which is great because we often have precious gDNA samples. We also used it to amplify directly off serial dilutions of lymphoblast cell line suspensions. We found we were able to amplify off of between 300 and 500 cells direct. We're very happy with that aspect as well.
Researcher at a prestigious Genome Centre in Canada
KAPA2G Robust with separate vial of dNTP (250uL of 10mM each) 100 Units Recommend 0.5unit per 25uL reaction
Kit contents - 100 units, 5 U/µL. Supplied with 5X KAPA2G Buffer A, 5X KAPA2G Buffer B, 5X KAPA2G GC Buffer (all with Mg2+ at a 1X conc. of 1.5 mM), 5X KAPA Enhancer 1, dNTP Mix (10 mM) and extra MgCl2 (25 mM).
Streamline your PCR workflows by consolidating protocols and reaction conditions, increase pass rates, amplify difficult templates without bias, and increase sensitivity – all with a single enzyme.
The second-generation KAPA2G Robust DNA Polymerase was evolved to solve - achieve consistent amplification across a broad range of amplicon types (both GC and AT-rich). The versatility and robustness of the polymerase allows for consolidation of PCR cycling protocols and reaction conditions while increasing success rates. The high performance of the KAPA2G Robust DNA Polymerase eliminates the need for multiple enzymes and protocols - standardize your PCRs with a single enzyme solution.
The KAPA2G Robust DNA Polymerase offers:
Robust performance across a wide range of GC- and AT-rich templates
Increased PCR success rates
Consolidated PCR protocols and reaction conditions with a single enzyme
Improved tolerance to common PCR inhibitors for crude samples and/or DNA extractions
Consistent Colony PCR from E. coli and yeast
Higher yield and sensitivity per unit of enzyme
Three novel buffers and a proprietary PCR enhancer offer extended optimization options for the most difficult templates
KAPA2G Robust DNA Polymerase is also available with HotStart technology for improved specificity during prolonged benchtop setup or liquid handling.
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