Anachem Ltd. is THE pipette & laboratory supplies specialist in the UK & Ireland. Founded in 1970, our 40 years of exclusive distribution ensures we have the best expertise in pipetting technical support, maintenance & calibration, customer service & e-commerce. We are the people you can trust and rely on for all pipettes, pipettes tips, pipette calibration, general laboratory & PCR plastics, plus innovative new molecular biology reagents. Anachem is now a METTLER TOLEDO company.
Anachem's 40 years of expertise in pipetting makes it THE supplier of choice for an extensive range of the highest quality pipetting products from leading manufacturers such as RAININ. For all types of liquid handling tools: micropipette, volumetric pipette, automatic pipette, multichannel pipette, electronic pipette, bottle top dispenser, 96-well dispenser- they are all here.
The highest quality pipette tips including the Rainin BioClean line ensures you get maximum performance and no interference in your assays. Anachem provides innovative packaging systems for disposable tips that minimize environmental impact. Our tips fit a wide range of pipettes - get help finding the tips you need with our Pipette Tip Selector.
Sample Storage: our extensive range of high quality tubes, storage racks and labelling materials ensure your precious samples are protected, maintain their integrity and are easy to locate. From graduated 0.5ml microtubes and vials up to 15ml and 50ml centrifuge tubes, they are all made from the purest polypropylene.
96 well, 384 well and deep well microplates for your microtiter and high throughput applications. For PCR - diagnostic, quatitative or real time our PCR plates and PCR tubes are made from the purest materials to protect your samples and eliminate assay interference. Innovative features enhance sensitivity and detection levels of dna amplification. New 4titude plates offer superior performance at great value.
Protect yourself, your lab equipment and your samples against the safety hazards exposed to in your laboratory work. Anachem laboratory supplies provides effective decontamination solutions such as MicroSol3plus & Virkon for general disinfection and any microbiology lab. Protective gloves, trays and radiation safety shields also support your laboratory procedures in biological or chemical areas.
Key reagents and instrumentation for nucleic acid manipulations, DNA extraction, PCR & electrophoresis. New KAPA Biosystems Second Generation PCR polymerases bring advanced performance and yield, with specialist enzymes for real time PCR with SYBR® Green, Hot Start PCR etc.
Liquidator®96 Manual 96-well Pipettor provides simultaneous pipetting into 96 wells with accuracy and efficiency. Liquidator 96 is designed to maximize workflow without the need for complicated programming or dedicated technician time. Incredibly fast and adds outstanding quality features to high throughput pipetting with many applications.
With precise results, easy cleaning and a design made to last, METTLER TOLEDO NewClassic balances help you deal with many scientific challenges, from boosting productivity, achieving high quality, to serving heightened customer demands. Swiss technology, precision and quality assist you in creating lasting value for your laboratory.
Whether you need solutions for complex applications under regulatory control or for simple routine measurements, METTLER TOLEDO’s comprehensive pH and conductivity range covers varied applications across all sectors. Backed by 60 years of expertise in sensor innovation and an extensive range of accessories, you can be assured of a product range of unparalleled quality and flexibility.
Anachem is the largest and most experienced pipette calibration & service organisation in the UK and Ireland. With over 30 years of expertise in all aspects of pipette maintenance for all makes and models there is no better support to ensure your pipette accuracy and precision. We have held UKAS accreditation for pipette calibration since 1998.
Our library quantification issues are solved. We can now multiplex our libraries and get the right proportions of reads!
Quantification Method
Concentration
First nanodrop
177 nM
Second nanodrop
69 nM
Qubit
30 nM
Bioanalyzer
4.7 nM
Kapa Library Quant qPCR
4.2 nM
Dr C Duncan, Cambridge University, 01 May 2013
I used KAPA HiFi HS to amplify a 7kb component of the nuclear pore complex, which I had difficulties getting a clean amplification. The result was very satisfying and superior to all the other high-fidelity, proof-leading polymerases used in our lab.
Dr P Louphrasitthiphol, Oxford University, 29 January 2013
KAPA Library Quantification Kits For Roche 454 GS FLX Platform includes 1 x KK4601 KAPA SYBR Fast Universal qPCR Kit and 1 x KK4800 1x1ml 454 FLX Primer Premix (10x) and 6x80µl 454 FLX DNA Standards
KAPA Library Quant Kits
Now available for Ion Torrent PGM!
Lessen time and costly titrations and reduce variability in cluster density or template-to-bead ratio with a high performance qPCR solution for next-gen sequencing library quantification.
Regular methods for calculating next-generation sequencing libraries have a number of important disadvantages. Electrophoresis and spectrophotometry measure total nucleic acid concentrations, whereas optimal cluster density or template-to-bead ratio depend on the appropriate concentration of PCR-amplifiable DNA molecules. These methods also have low sensitivity, consuming nanograms of precious samples, and are not suitable for high-throughput workflows.
Quantitative PCR (qPCR) is inherently well-suited for next-generation sequencing library quantification:
qPCR specifically quantifies only PCR-competent DNA molecules,
is highly sensitive allowing accurate quantification of low concentration libraries,
is amenable to automated liquid handling.
KAPA Library Quantification Kits are optimised for the Illumina Genome Analyzer, Roche 454 Titanium series, and Roche 454 FLX series platforms and include highly reproducible and precisely defined sets of serially diluted DNA concentration standards and state-of-the-art qPCR reagents, which contain a DNA polymerase specifically engineered for robust, SYBR® Green I-tolerant amplification of long and difficult templates.
Please Call +44 (0)1582 455135 for stock availability
