Serves as molecular weight standard for electrophoresis for agarose gels. Suitable for the sizing of double-stranded DNA fragments. The lambda DNA is completely digested with EcoRI and HindIII and yields 12 fragments whose sizes are as follows:
564bp
831bp
947bp
1375bp
1584bp
1904bp
2027bp
3530bp
4268bp
4973bp
5148bp
21226bp
Store at -20°C
The ladder is stored at a concentration 0.1µg/µl in a buffer of 10mM Tris-HCl (pH 8.0) and 1mM EDTA.
Usage Recommendation
Use 0.5µg of the DNA marker per 1mm width of gel lane.