Serves as molecular weight standard for electrophoresis for agarose gels. Suitable for the sizing of double-stranded DNA fragments. The lambda DNA is completely digested with Hind III and yields 8 fragments whose sizes are as follows:
125bp
564bp
2027bp
2322bp
4361bp
6557bp
9416bp
23130bp
Store at -20°C
The ladder is stored at a concentration 0.1µg/µl in a buffer of 10mM Tris-HCl (pH 8.0) and 1mM EDTA.
Usage Recommendation
Use 0.5µg of the DNA marker per 1mm width of gel lane.