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> Home > Product Catalogue > Molecular Biology > PCR Enzymes > Elong8 KikStart Taq > KeyZyme KikStart Elong8 Taq Polymerase 200 Units
AM-4501
KeyZyme KikStart Elong8 Taq Polymerase 200 Units
> Save 15% on KeyZyme PCR Enzymes & dNTP's - Offer ends 31/12/08
List Price
£88.00
Availability: Sorry Currently Not in Stock, expected on 01. November 2008
Quantity  
Kits contain 200u Keyzme KikStart Elong8 Taq for "Hot Start" PCR protocols with amplification range up to 20Kb 1ml 10X KeyZyme BufferA 1ml 10X KeyZyme BufferS and 1ml 50mM MgCl2

KeyZyme KikStart Elong8 Taq polymerase is a mixture of thermostable Taq DNA polymerase, proofreading Pfu DNA polymerase, anti-Taq DNA polymerase antibodies, reversible inhibitors and enhancers for automatic "Hot Start" PCR. It exhibits the 3' to 5' proofreading activity, resulting in considerably higher amplification fidelity than possible with unmodified Taq DNA polymerase. Recommended for use in amplification to obtain DNA products up to 20kb with stringent amplification specificity, sensitivity, fidelity and yield.

Switching to KeyZyme offers high quality PCR at reduced costs. The enzymes quality and perfomance is measured by sensitivity, consistency of manufacture plus competitor comparison.

Features

  • Ultra pure recombinant protein is reversibily complexed with an anti-Taq monoclonal antibody that blocks replication activity of the enzyme at moderate temperatures.
  • Excellent for multiple amplification as it exhibits wider tolerance for Mg2+ and salt concentrations.
  • Improves amplification results with critical templates, such as those containing GC-rich regions, palindromes or multiple repeats.

    Buffer Composition

    10X KeyZyme Buffer A (without MgCl2) : 500Mm KCl, 100mM Tris-HCl (pH 9.1 at 20°C) and 0.1% Triton™ X-100. The buffer is optimised for use with 0.1-0.2mM of each dNTP.

    10x Keyzyme Buffer S: 160mM (NH4)2SO4, 500mM Tris-HCl (pH 9.2 at 22°C), 17.5mM MgCl2 and 0.1% Triton™ X-100. The buffer is optimised for use with 0.35mM of each dNTP.

    Storage Buffer : 20mM Tris-HCl (pH 8.0 at 22°C), 100mM KCl, 0.5% Tween™20, 0.5% Nonidet-P40, 0.1mM EDTA and 50% glycerol.

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