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> Home > Product Catalogue > Molecular Biology > PCR Enzymes > KikStart Taq > KeyZyme KikStart Taq Polymerase 500 Units
AM-4402
KeyZyme KikStart Taq Polymerase 500 Units
List Price
£175.00
In Stock
Quantity  
Kits contain 200u Keyzyme KikStart Taq for "Hot-Start" PCR protocols 2ml 10X KeyZyme BufferA 1ml 10X KeyZyme BufferS and 1ml 50mM MgCl2

KeyZyme KikStart Taq DNA polymerase is a complex of specific anti-Taq monoclonal antibody with top quality thermostable Taq DNA polymerase for automatic "Hot Start" amplification, resulting in greatly enhanced amplification specificity. KeyZyme KikStart Taq DNA polymerase catalyzes the polymerisation of nucleotides into duplex DNA in the 5' to 3' direction with presence of Mg2+ but maintains the 5' to 3' exonuclease activity.

Switching to KeyZyme offers high quality PCR at reduced costs. The enzymes quality and perfomance is measured by sensitivity, consistency of manufacture plus competitor comparison.

Features

  • Ultra pure recombinant protein that is reversibly complexed with an anti-Taq monoclonal antibody which blocks the replication activity of the enzyme at moderate temperatures.
  • Carefully selected anti-Taq antibodies have high thermal stability, providing protection against non-specific primer extension from room temperature to 80°C
  • Formation of complexes between Taq DNA polymerase and an anti-Taq antibody forms a basis for automatic "hot start" amplification, which allows for the assembly of amplification reactions at room temperature.
  • High stability of the complexes allows for the enormous increase in amplification specificity, sensitivity and yield in comparison to the convential amplification assembly method.
  • Increased specificity as a result of reduced amplification artifacts such as primer-dimer formation and mispriming in multiplex amplification.

    Kit Contents

    2ml of 10X Buffer A

    1ml of 10X Buffer S

    1ml of 50mM MgCl2

    Buffer Composition

    10X KeyZyme Buffer A (without MgCl2) : 500mM KCl, 100mM Tris-HCl (pH 9.1 at 20°C) and 0.1% Triton™ X-100. The buffer is optimised for use with 0.1-0.2mM of each dNTP.

    10x Keyzyme Buffer S: 160mM (NH4)2SO4, 500mM Tris-HCl (pH 9.2 at 22°C), 17.5mM MgCl2 and 0.1% Triton™ X-100. The buffer is optimised for use with 0.35mM of each dNTP.

    Storage Buffer : 20mM Tris-HCl (pH 8.0 at 22°C), 100mM KCl, 0.5% Tween™20, 0.5% Nonidet-P40, 0.1mM EDTA, 1mM DTT and 50% glycerol.

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