You are Viewing Prices in GBP View Prices in Euros
> Home > Product Catalogue > Molecular Biology > PCR > Enzymes > AKUR8 Taq > Keyzyme AKUR8 Pfu Polymerase 500 Units
AM-4302
Keyzyme AKUR8 Pfu Polymerase 500 Units
>
List Price
£138.00
In Stock
Quantity  
Keyzyme AKUR8 PFU is for high fidelity PCR and cloning blunt end PCR products with 10 X higher fidelity than standard Taq. This kit contains 500 units of Pfu and buffers and MgCl2

KeyZyme AKUR8 Pfu DNA polymerase is an extremely thermostable proofreading DNA polymerase, suuitable for applications requiring high temperature synthesis of DNA. KeyZyme AKUR8 Pfu DNA polymerase catalyzes the polymerisation of nucleotides into duplex DNA in the 5' to 3' direction with the presence of Mg2+. It exhibits the 3' to 5' proofreading activity.

Switching to KeyZyme offers high quality PCR at reduced costs. The enzymes quality and perfomance is measured by sensitivity, consistency of manufacture plus competitor comparison.

Features

  • Ultra pure recombinant protein.
  • Recommended for use in high fidelity amplification of GC-rich sequences or problematic secondary structures, primer extension reactions at elevated temperatures and cloning of blunt-ended amplification products.
  • Proof reading, 10 fold higher fidelity than Taq.

    Unit Definition

    1u is defined as the amount of enzyme that is required to catalyze the incorporation of 10nmol of dNTP into acid-insoluble material in 30 minutes at 74°C.

    Buffer Composition

    10X KeyZyme Buffer A (without MgCl2) : 500Mm KCl, 100mM Tris-HCl (pH 9.1 at 20°C) and 0.1% Triton™ X-100. The buffer is optimised for use with 0.1-0.2mM of each dNTP.

    10x Keyzyme Buffer S: 160mM (NH4)2SO4, 500mM Tris-HCl (pH 9.2 at 22°C), 17.5mM MgCl2 and 0.1% Triton™ X-100. The buffer is optimised for use with 0.35mM of each dNTP.

    Storage Buffer : 20mM Tris-HCl (pH 8.0 at 22°C), 100mM KCl, 0.5% Tween™20, 0.5% Nonidet-P40, 0.1mM EDTA, 1mM DTT, color dyes and 50% glycerol.

    		•