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£62.00
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£52.70
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Kits contain 500u Elong8 Keyzyme Taq with an amplification range up to 20Kb 2.5ml 10X KeyZyme BufferA 2.5ml 10X KeyZyme BufferS and 12.5ml 50mM MgCl2
KeyZyme Elong8 Taq DNA polymerase is a modified and optimised thermostable enzyme blend containing Taq DNA polymerase, Pfu DNA polymerase and enhancing factors. It exhibits the 3' to 5' proofreading activity, resulting in considerably higher fidelity than possible with unmodified Taq DNA polymerase. Recommended for use in amplification to obtain DNA products up to 20kb.
Switching to KeyZyme offers high quality PCR at reduced costs. The enzymes quality and perfomance is measured by sensitivity, consistency of manufacture plus competitor comparison.
Features
Ultra pure recombinant protein
Excellent for multiplex amplification as it exhibits wider tolerance for Mg2+ and salt concentrations.
Improves amplification results with critical templates, such as those containg GC-rich regions, palindromes or multiple repeats.
Increased amplification product yields and purity
Generates a mixture of blunt end and 3' dA overhang amplification products, majority of the products are blunt ended.
Buffer Composition
10X KeyZyme Buffer A (without MgCl2) : 500mM KCl, 100mM Tris-HCl (pH 9.1 at 20°C) and 0.1% Triton™ X-100. The buffer is optimised for use with 0.1-0.2mM of each dNTP.
10x Keyzyme Buffer S: 160mM (NH4)2SO4, 500mM Tris-HCl (pH 9.2 at 22°C), 17.5mM MgCl2 and 0.1% Triton™ X-100. The buffer is optimised for use with 0.35mM of each dNTP.
Storage Buffer : 20mM Tris-HCl (pH 8.0 at 22°C), 100mM KCl, 0.5% Tween™20, 0.5% Nonidet-P40, 0.1mM EDTA and 50% glycerol.
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