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£24.00
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£20.40
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Kits contain 200u Keyzyme Taq with color indicator 2ml 10X KeyZyme BufferA 1ml 10X KeyZyme BufferS and 1ml 50mM MgCl2
KeyZyme Taq DNA polymerase is a thermostable DNA polymerase. It comes from an E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus. It is suitable for applications requiring high temperature synthesis of DNA. Taq DNA polymerase catalyzes the polymerisation of nucleotides into duplex DNA in the 5' to 3' direction with presence of Mg2+ but maintains the 5' to 3' exonuclease activity. The enyzme is supplemented with with an inert colour dye.
Switching to KeyZyme offers high quality PCR at reduced costs. The enzymes quality and perfomance is measured by sensitivity, consistency of manufacture plus competitor comparison.
Features
Thermostable enzyme of approximately 94kDa from Thermus aquaticus.
Ultra pure recombinant protein.
Replicate DNA at 74°C and exhibits a half-life if 40 minutes at 96°C
Generates mostly 3' dA overhang PCR products which are suitable for TA cloning.
Kit Contents:
500u of KeyZyme Color Taq (1µ/µl)
2ml of 10X Buffer A
1ml of 10X Buffer S
1ml of 50mM MgCl2
Buffer Composition
10X KeyZyme Buffer A (without MgCl2) : 500Mm KCl, 100mM Tris-HCl (pH 9.1 at 20°C) and 0.1% Triton™ X-100. The buffer is optimised for use with 0.1-0.2mM of each dNTP.
10x Keyzyme Buffer S: 160mM (NH4)2SO4, 500mM Tris-HCl (pH 9.2 at 22°C), 17.5mM MgCl2 and 0.1% Triton™ X-100. The buffer is optimised for use with 0.35mM of each dNTP.
Storage Buffer : 20mM Tris-HCl (pH 8.0 at 22°C), 100mM KCl, 0.5% Tween™20, 0.5% Nonidet-P40, 0.1mM EDTA, 1mM DTT, color dyes and 50% glycerol.
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