Anachem Ltd. is THE pipette & laboratory supplies specialist in the UK & Ireland. Founded in 1970, our 40 years of exclusive distribution ensures we have the best expertise in pipetting technical support, maintenance & calibration, customer service & e-commerce. We are the people you can trust and rely on for all pipettes, pipettes tips, pipette calibration, general laboratory & PCR plastics, plus innovative new molecular biology reagents. Anachem is now a METTLER TOLEDO company.
Anachem's 40 years of expertise in pipetting makes it THE supplier of choice for an extensive range of the highest quality pipetting products from leading manufacturers such as RAININ. For all types of liquid handling tools: micropipette, volumetric pipette, automatic pipette, multichannel pipette, electronic pipette, bottle top dispenser, 96-well dispenser- they are all here.
The highest quality pipette tips including the Rainin BioClean line ensures you get maximum performance and no interference in your assays. Anachem provides innovative packaging systems for disposable tips that minimize environmental impact. Our tips fit a wide range of pipettes - get help finding the tips you need with our Pipette Tip Selector.
Sample Storage: our extensive range of high quality tubes, storage racks and labelling materials ensure your precious samples are protected, maintain their integrity and are easy to locate. From graduated 0.5ml microtubes and vials up to 15ml and 50ml centrifuge tubes, they are all made from the purest polypropylene.
96 well, 384 well and deep well microplates for your microtiter and high throughput applications. For PCR - diagnostic, quatitative or real time our PCR plates and PCR tubes are made from the purest materials to protect your samples and eliminate assay interference. Innovative features enhance sensitivity and detection levels of dna amplification. New 4titude plates offer superior performance at great value.
Protect yourself, your lab equipment and your samples against the safety hazards exposed to in your laboratory work. Anachem laboratory supplies provides effective decontamination solutions such as MicroSol3plus & Virkon for general disinfection and any microbiology lab. Protective gloves, trays and radiation safety shields also support your laboratory procedures in biological or chemical areas.
Key reagents and instrumentation for nucleic acid manipulations, DNA extraction, PCR & electrophoresis. New KAPA Biosystems Second Generation PCR polymerases bring advanced performance and yield, with specialist enzymes for real time PCR with SYBR® Green, Hot Start PCR etc.
Liquidator®96 Manual 96-well Pipettor provides simultaneous pipetting into 96 wells with accuracy and efficiency. Liquidator 96 is designed to maximize workflow without the need for complicated programming or dedicated technician time. Incredibly fast and adds outstanding quality features to high throughput pipetting with many applications.
With precise results, easy cleaning and a design made to last, METTLER TOLEDO NewClassic balances help you deal with many scientific challenges, from boosting productivity, achieving high quality, to serving heightened customer demands. Swiss technology, precision and quality assist you in creating lasting value for your laboratory.
Whether you need solutions for complex applications under regulatory control or for simple routine measurements, METTLER TOLEDO’s comprehensive pH and conductivity range covers varied applications across all sectors. Backed by 60 years of expertise in sensor innovation and an extensive range of accessories, you can be assured of a product range of unparalleled quality and flexibility.
Anachem is the largest and most experienced pipette calibration & service organisation in the UK and Ireland. With over 30 years of expertise in all aspects of pipette maintenance for all makes and models there is no better support to ensure your pipette accuracy and precision. We have held UKAS accreditation for pipette calibration since 1998.
I looked at the KeyPrep Plasmid DNA extraction kit because of the cost savings and have found these kits to be excellent. The yield of the plasmids is not only high compared to other commercial kits, but also produced very good sequencing results. Almost all of the sequencing results I obtained from pGEM-T originated plasmids, in DH5-a cells, isolated using the KeyPrep Plasmid DNA extraction kit, contained a minimum of 800 clean bases using M13F or M13R primers.
Ms B Chung, University College Cork, 06 February 2009
KeyPrep Spin Plasmid Mini Kit 100 Preps. Kits contain Buffers and Spin Columns required to purify up to 20ug High Copy or Low Copy Plasmid DNA from Bacterial cultures.
KeyPrep Nucleic Acid Mini Kits
The high quality kits you need at prices that are hard to beat
Anachem KeyPrep Spin columns have a unique low density glass filter membrane that ensures higher Nucleic Acid binding capacity, enhanced removal of impurities and increased NA recovery compared to conventional silica membrane formats used in competitive kits.
Customers see enhanced yields, sensitivity and purity compared to competitor kits.
KeyPrep Plasmid Kits are designed for rapid and efficient purification of high or low copy plasmid DNA without the need for precipitation or organic extractions. They contain Buffers and Spin Columns required for the purification of up to 20μg high copy or low copy Plasmid DNA from E.coli cultures.
The highly purified Plasmid DNA is then ready for use in routine molecular biology applications such as restriction enzyme digestion, sequencing, PCR, ligation, and transformation.
The expected Plasmid DNA yields and purity are shown below:
Culture Vol.
Yield
Purity
High Copy Number Plasmid (50-500 copies per cell)
2ml
2-5µg
1.7-1.9
5ml
10-20µg
1.7-1.9
Low Copy Number Plasmid (1-10 copies per cell)
2ml
1-3µg
1.7-1.9
5ml
5-10µg
1.7-1.9
KeyPrep benefits include:
· Quality
Purity and yields of high and low copy plasmids are comparable with or better than competitive kits. As can be seen on the gels above and the table of results below:
pTZ57R
pCAMBIA
pBR322
Ng/μl
A260/280
Ng/μl
A260/280
Ng/μl
A260/280
KeyPrep
135
1.98
KeyPrep
210
1.93
KeyPrep
63.5
1.97
Competitor P
1.92
1.79
Competitor P
133
1.91
Competitor P
68.3
1.97
Competitor Q
1.88
2.12
Competitor Q
174
1.90
Competitor Q
58.2
1.98
· Reliability and Reproducibility
Exhaustive membrane and Kit QC procedures ensure consistent prep to prep and kit to kit performance. Competitor comparisons are included within the routine QC procedures
· Cost Saving
KeyPrep kits offer savings of at least 25% compared to comparable market-leading products. Even greater savings can by setting up departmental or organisation-wide discount schemes.
· Speed
Alkaline lysis, neutralization and centrifugation to remove the lysed cells are the most time consuming part of any plasmid prep (17 minutes). The subsequent binding, washing and eluting steps should take less than 5 – 10 minutes depending on the number of mini preps being done.
· Technical Support
Anachem offers excellent customer support for all its products. Technical support can be contacted by phone, 01582 747 500, or by email, mbtechnical@anachem.co.uk.
· No Risk Trial
If you order a kit and it does not perform to specification, contact us within 30 days and we will refund you the price you paid for the kit.
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