KeyPrep Spin Bacterial Genomic DNA Kit 100 preps
AM-111113
KeyPrep Spin Bacterial Genomic DNA Kit 100 preps
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£ 235.30
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I used KAPA HiFi HS to amplify a 7kb component of the nuclear pore complex, which I had difficulties getting a clean amplification. The result was very satisfying and superior to all the other high-fidelity, proof-leading polymerases used in our lab.

 


Dr P Louphrasitthiphol, Oxford University, 29 January 2013


The Kapa Illumina library quantification kit is easy to use and allows all types of Illumina libraries to be quantified quickly.

The kit contains a primer premix that is directly added to the 2x Kapa SYBR Fast qPCR Master Mix before use. Having the master mix already prepared helps to dramatically reduce the time spent setting up each qPCR plate. The kit also contain 6 DNA standards which allow quantification of library dilutions up to a concentration of 20 pM.

This kit has greatly helped us to create reproducible cluster numbers on each Illumina NGS run and platform used and is a real necessity for anyone quantifying Illumina libraries.
 


Dr S Holden, Manchester University, 20 November 2012




Keyprep Bacterial Genomic Mini Kit 100 Preps. Kit contains Buffers and Spin columns to purify up to 20ug high molecular weight genomic DNA from Bacterial Cultures

KeyPrep Nucleic Acid Mini Kits

The high quality kits you need at prices that are hard to beat

Anachem KeyPrep Spin columns have a unique low density glass filter membrane that ensures higher Nucleic Acid binding capacity, enhanced removal of impurities and increased NA recovery compared to conventional silica membrane formats used in competitive kits.

Customers see enhanced yields, sensitivity and purity compared to competitor kits.

KeyPrep Bacterial Genomic Kits are designed for rapid and efficient purification genomic DNA from Gram-positive and Gram-negative bacteria. They contain buffers and spin columns required for purification of up to 20μg genomic DNA from 1 – 3 ml bacterial cultures.
The highly purified genomic DNA is then ready for use in routine molecular biology applications such as restriction enzyme digestion, PCR, Southern Blotting, DNA fingerprinting and other manipulations.
KeyPrep Benefits
· Quality
Purity and yields of genomic DNA from bacteria are comparable with or better than competitive kits. KeyPrep has been shown to be effective across a range of Gram-negative and Gram-positive bacteria.
· Reliability and Reproducibility
Exhaustive membrane and Kit QC procedures ensure consistent prep to prep and kit to kit performance. Competitor comparisons are included within the routine QC procedures.
· Cost Saving
KeyPrep kits offer savings of at least 25% compared to comparable market-leading products. Even greater savings can be made by setting up departmental or organisation-wide discount schemes.
· Speed
The initial 20 minute Lysozyme incubation and 20 minute Protein Denaturation steps are the most time consuming steps of any Bacterial Genomic DNA prep. The subsequent binding, washing and eluting steps should take less than 5 – 10 minutes depending on the number of mini preps being done.
· Technical Support
Anachem offers excellent customer support for all its products. Technical support can be contacted by phone, 01582 747 500, or by email, mbtechnical@anachem.co.uk.
· No Risk Trial
If you order a kit and it does not perform to specification, contact us within 30 days and we will refund you the price you paid for the kit.
Kit Components
Product
Catalogue Number
50 Preps
AM-111112
100 Preps
AM-111113
KeyPrep Spin Columns
50
100
Collection Tubes
50
100
Re-suspension Buffer 1 (Buffer R1)
12ml
24ml
Re-suspension Buffer 1 (Buffer R1)
15ml
30ml
Bacterial Genomic DNA Binding Buffer (Buffer BG)
25ml
50ml
Wash Buffer (Concentrate)*
5ml
10l
Elution Buffer
5ml
10ml
Proteinase K
1ml
2ml
Hand Book
1
1
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