I tried countless combinations of qPCR reagents, buffers and primer sets before contacting KAPA Biosystems for a sample of their KAPA SYBR® FAST qPCR Kit (Universal), somewhat in desperation.
I had immediate success and have never looked back. I trust the ability of the enzyme to generate reliable results quick and simply, typically without optimisation. I refuse to use other reagents now.
Dr. Phillippa Taberlay, Ph.D - The Garvan Institute of Medical Research