Our library quantification issues are solved. We can now multiplex our libraries and get the right proportions of reads!
| Quantification Method |
Concentration |
| First nanodrop |
177 nM |
| Second nanodrop |
69 nM |
| Qubit |
30 nM |
| Bioanalyzer |
4.7 nM |
| Kapa Library Quant qPCR |
4.2 nM |
Dr C Duncan, Cambridge University, 01 May 2013
I have been using KAPA SYBR reagents for the last year to study alterations in gene expression in Squamous Cell Carcinoma using an ABI 7500 realtime PCR system. KAPA SYBR reagents have allowed me to produce robust reproducible data validating microarray, protein array and western blot data at the RNA level.
Dr M Caley, Queen Mary University of London, 11 February 2013
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