Robust PCR

Streamline your PCR workflows by consolidating protocols and reaction conditions, increase pass rates, amplify difficult templates without bias, and increase sensitivity – all with a single enzyme.

KAPA2G Robust DNA Polymerase

KAPA2G Robust DNA Polymerase

Achieve consistent amplification across a broad range of amplicon types (both GC and AT-rich). The high performance of the KAPA2G Robust DNA Polymerase eliminates the ...

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KAPA2G Robust HotStart DNA Polymerase

KAPA2G Robust HotStart DNA Polymerase

HotStart technology for improved specificity during prolonged benchtop setup or liquid handling.
Customer Reviews

I have been using the KAPA SYBR FAST qPCR kits for more than 2 years.

The qPCR kits gave equal and in many cases better qPCR performance on the ABI HT7900, PikoReal 96 and the BioRad CFX-384 qPCR platforms compared to kits from other manufacturers.

Pricing is really good (cheapest on the market by far) so we’ve been able to save money.

The KAPA SYBR FAST qPCR kits is very simple to use so there's no need to optimise qPCR protocol or conditions if switching over from kits of other manufacturers.

Mr L Luong, Queen Mary University of London, 19 March 2014


Ease of Use
Value for Money


Our library quantification issues are solved. We can now multiplex our libraries and get the right proportions of reads!

Quantification Method Concentration
First nanodrop 177 nM
Second nanodrop 69 nM
Qubit 30 nM
Bioanalyzer 4.7 nM
Kapa Library Quant qPCR 4.2 nM

 

Dr C Duncan, Cambridge University, 01 May 2013



I have been using KAPA SYBR reagents for the last year to study alterations in gene expression in Squamous Cell Carcinoma using an ABI 7500 realtime PCR system. KAPA SYBR reagents have allowed me to produce robust reproducible data validating microarray, protein array and western blot data at the RNA level.

Dr M Caley, Queen Mary University of London, 11 February 2013


Ease of Use
After Sales Service
Value for Money


I used KAPA HiFi HS to amplify a 7kb component of the nuclear pore complex, which I had difficulties getting a clean amplification. The result was very satisfying and superior to all the other high-fidelity, proof-leading polymerases used in our lab.

 

Dr P Louphrasitthiphol, Oxford University, 29 January 2013



The Kapa Illumina library quantification kit is easy to use and allows all types of Illumina libraries to be quantified quickly.

The kit contains a primer premix that is directly added to the 2x Kapa SYBR Fast qPCR Master Mix before use. Having the master mix already prepared helps to dramatically reduce the time spent setting up each qPCR plate. The kit also contain 6 DNA standards which allow quantification of library dilutions up to a concentration of 20 pM.

This kit has greatly helped us to create reproducible cluster numbers on each Illumina NGS run and platform used and is a real necessity for anyone quantifying Illumina libraries.
 

Dr S Holden, Manchester University, 20 November 2012


Ease of Use


I tried countless combinations of qPCR reagents, buffers and primer sets before contacting KAPA Biosystems for a sample of their KAPA SYBR® FAST qPCR Kit (Universal), somewhat in desperation.
 

I had immediate success and have never looked back. I trust the ability of the enzyme to generate reliable results quick and simply, typically without optimisation. I refuse to use other reagents now.

 

Dr. Phillippa Taberlay, Ph.D - The Garvan Institute of Medical Research

 

13 December 2011


Ease of Use
After Sales Service
Value for Money


“I used DNAReleasy on C. elegans and was really impressed with the results; DNAReleasy lysed the worms in just 7 minutes!”
Research Fellow – University College London

08 September 2010


Ease of Use
After Sales Service
Value for Money


"Before qPCR was adopted for library quantification, cluster density was extremely variable. Implementation of the KAPA Library Quantification Kit into our sequencing workflows resulted in a significant reduction in variability across multiple libraries, negating the need for cluster amplification titration runs".
- The Broad Institute, Cambridge, MA U.S.A.
 

 

04 August 2010



I have now tried the KAPA HiFi HotStart enzyme and sequenced cloned PCR fragment to see how many introduced PCR mutation this enzyme gives.

Template: cDNA from the C2C12 cell line

Expected product size: 2.3 kb

Reaction setup: As described in the manufacturers manual

Cycles:

95° 3'
98° 20" 60° 15" 72° 90" 40x
72° 3'

Cloned and sequenced three individual clones.

Mutation: 0 (zeeeeero)

I have also used this enzyme for low complexity amplifications (plasmid as template and 16 cycles) and have not seen a single introduced mutation.

KAPA HiFi HotStart is absolutely the best enzyme I have ever used when high fidelity is desired and is my future choice for all my PCR applications, instead of the many other enzymes I have used and tried over nearly two decades in science.

KAPA HiFi HotStart has also, in my hands, proven to be very robust; it has worked every time even with known difficult template and primer pairs.


Göran Hjälm, Assc. Professor
Uppsala University
Sweden

06 January 2010


Ease of Use
After Sales Service
Value for Money


The KAPA HiFi and KAPA HiFi Hotstart DNA Polymerases are really amazing! At first, I and my lab mate hesitate to use these products since we are not familiar with it and we are afraid to take the risk considering that we just have few DNA samples and a limited time to finish our work. However, our lack of confidence to these products leads us to be delayed. We have used Hifi DNA Polymerases from some renowned companies like Invitrogen, Promega, Roche and Stratagene for a long period of time but we never got such outstanding result. HiFi DNA Polymerases from KAPA were able to amplify our desired fragment up to 9Kb. The bands produced were so intense and there were no extra or any unspecific bands. Furthermore, these DNA Polymerases need no optimization. This is excellent, making PCR easier, more interesting and exciting!

Jeanette Lescano-Balindong, Researcher
Plant Breeding, Genetics and Biotechnology
International Rice Research Institute, Manilla

 

25 August 2009



I had been trying to amplify a GC rich region of genomic DNA with poor quality samples. I tested several enzymes including Amplitaq 360, Thermo-Start, Herculase II and HotStar, but without much success. I was very impressed with the results of the Kapa Biosystems Robust PCR Kit, as it has given me much better amplification than any other enzyme and combination of buffers I have tried.

04 August 2009


Ease of Use
After Sales Service
Value for Money


We switched to Kapa Biosystems SYBR Fast qPCR kit because we noticed a significant improvement in PCR efficiency and steepness of amplification curves.

I work for the Microarray Core facility of the University of Basel, Switzerland. We lead genome-wide studies on the RNA (gene expression and alternative splicing) and the DNA (SNPs and Copy Number Variation). Real-time PCR is a key player for validating our results. We own a Corbett Research Rotor-Gene 3000A. After using various SYBR Green Kits over the past years we switched to SyBr Fast kit from Kapa Biosystems because we noticed a significant improvement in PCR efficiency, and especially in steepness of amplification curves. What I appreciate the most is the fact this kit works very nicely for the vast majority of primers we design without optimization and for various applications (Gene expression as well as DNA Copy Number). This universality makes our real-time PCR assays very fast to establish and our results more reliable.

 

Philippe Demouging, Molecular Psychology, University of Basel
 

04 June 2009



I had been experiencing poor yield attempting to amplify with Expand, a product that should have been trivial -- a 200-bp product from a plasmid template. So, I tried all 10 PCR enzymes we have in the lab with the same primers and template and the same cycling parameters (a rather generic program, optimized for none of the enzymes) using the basic buffer conditions for each enzyme, and estimated the yield by running a portion of the reactions on a gel.
The rankings, by yield:
1) Invitrogen Amplitaq (low fidelity) in Roche Taq buffer (however, a non-specific band was also amplified)
2) (TIE) Pfx50 and Kapa HiFi
3) Pfu Ultra
4) Pfu Turbo
5) Phusion
6) Expand plus 5% DMSO (since it didn't work well without DMSO in previous trials)
7) Qiagen Taq (a non-specific band was also amplified) Pfu and Pfx failed to amplify any detectable product.
For a high-fidelity application (like this test example), I'd choose Kapa HiFi. So far so good!
 

Scientist at the University of California at Berkley

04 June 2009



I tested four samples with both ABI and KAPA SYBR FAST reagents. The KAPA SYBR reagent can bring down the Ct values by 1.5-2, which is amazing. I will go ahead and use it for my future CHIP experiments.

Junqi Song, Duke University

04 June 2009



I tried out the Kapa2G robust kit. We're super happy with it. We first tested it on gDNA and the amount of template needed was very minimal, 2ng in a 25ul reaction, which is great because we often have precious gDNA samples. We also used it to amplify directly off serial dilutions of lymphoblast cell line suspensions. We found we were able to amplify off of between 300 and 500 cells direct. We're very happy with that aspect as well.


Researcher at a prestigious Genome Centre in Canada

 

06 April 2009



I tested the new Bambanker system from Anachem with my Calu-3 cell line. I was very pleased with the results I obtained from the Bambanker and could see an increase in yield of approximately 20%. I found the system to be extremely easy to use as I didn’t need to perform any dilutions or use any additives. It is very simple, I just added Bambanker to my cells and placed them into the -80ºC freezer before transferring to liquid nitrogen the next day.

16 February 2009



I looked at Anachem's Taq Mastermix because of the cost savings and its ease of use. I used the Taq Mastermix for colony screening and found it to be very efficient. Not only are the bands clear but, in my case, there are hardly any false positives.

16 February 2009


Ease of Use
After Sales Service
Value for Money


At the Department of Surgery, Royal Free Hospital London we were pleased to evaluated the new serum-free medium BAMBANKER™ that’s now available in the UK from Anachem Ltd.

Our first trial was with primary human aortic smooth muscle cells that were frozen in accordance with the operating procedure for Bambanker, stored at -80oC and successfully revived a week later. Our second test was with a SW620 human colon cancer cell line, which again was frozen in accordance with the operating procedure and stored at -80oC for a few days. The cells were again successfully revived.

We did not calculate the % recovery but we were very happy with the resulting cells after revival. Because of these trials, we feel that the advantages of the simple method employed and good cell recovery achieved with the Bambanker solution are considerable and we will definitely be using it from now on to freeze all our cells in the cell bank.
 

Dr G Punshon, University College London, 11 February 2009



I have recently changed from my Roche Extraction Kit to the Anachem Gel Recovery Kit (AM-113163) and I am delighted with the results I am getting. The Anachem kit gives us intense bands and great results every time. And as an added bonus we can use this kit for both gel extraction and PCR clean up, saving us money and lab space as we don’t need two different kits.

Ms L Draper, University College Cork, 10 February 2009


Ease of Use
After Sales Service
Value for Money


I looked at the KeyPrep Plasmid DNA extraction kit because of the cost savings and have found these kits to be excellent. The yield of the plasmids is not only high compared to other commercial kits, but also produced very good sequencing results. Almost all of the sequencing results I obtained from pGEM-T originated plasmids, in DH5-a cells, isolated using the KeyPrep Plasmid DNA extraction kit, contained a minimum of 800 clean bases using M13F or M13R primers.

06 February 2009


Ease of Use
After Sales Service
Value for Money


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