Product Catalogue

I have now tried the KAPA HiFi HotStart enzyme and sequenced cloned PCR fragment to see how many introduced PCR mutation this enzyme gives.

Template: cDNA from the C2C12 cell line

Expected product size: 2.3 kb

Reaction setup: As described in the manufacturers manual

Cycles:

95° 3'
98° 20" 60° 15" 72° 90" 40x
72° 3'

Cloned and sequenced three individual clones.

Mutation: 0 (zeeeeero)

I have also used this enzyme for low complexity amplifications (plasmid as template and 16 cycles) and have not seen a single introduced mutation.

KAPA HiFi HotStart is absolutely the best enzyme I have ever used when high fidelity is desired and is my future choice for all my PCR applications, instead of the many other enzymes I have used and tried over nearly two decades in science.

KAPA HiFi HotStart has also, in my hands, proven to be very robust; it has worked every time even with known difficult template and primer pairs.

Göran Hjälm, Assc. Professor
Uppsala University
Sweden

The KAPA HiFi and KAPA HiFi Hotstart DNA Polymerases are really amazing! At first, I and my lab mate hesitate to use these products since we are not familiar with it and we are afraid to take the risk considering that we just have few DNA samples and a limited time to finish our work. However, our lack of confidence to these products leads us to be delayed. We have used Hifi DNA Polymerases from some renowned companies like Invitrogen, Promega, Roche and Stratagene for a long period of time but we never got such outstanding result. HiFi DNA Polymerases from KAPA were able to amplify our desired fragment up to 9Kb. The bands produced were so intense and there were no extra or any unspecific bands. Furthermore, these DNA Polymerases need no optimization. This is excellent, making PCR easier, more interesting and exciting!

Jeanette Lescano-Balindong, Researcher
Plant Breeding, Genetics and Biotechnology
International Rice Research Institute, Manilla