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The Liquidator Manual 96 well Pipetting System brings Flexibility and Productivity to the Genomics Workflow

This interesting case study describes the involvement of the Canadian Centre for DNA Barcoding (CCDB) in the 'International Barcode of Life' project.

The project is developing a reference database of short standardised gene regions. The aim is to identify and discover 5 million specimens from 500, 000 species over the next five years.

Since 2009 the CCDB has processed approximately 300, 000  samples per year from animal, plant and fungal sources.

Samples follow a typical Genomics Workflow, with DNA extraction and PCR amplification. For animals downstream analysis methods include sequencing DNA barcodes comprising 650 bp.
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In this video you can follow the work of Dr. Natalia Ivanova, Lead DNA Scientist, and see how the Rainin Liquidator 96 plays a fundamental role in the numerous liquid handling procedures required to process her DNA samples.

Application 1: DNA Extraction from Animal and Plant samples

A typical day involves processing 20-40 plates using a variety of sample sources. For robotic DNA extraction, the Liquidator 96 is used to set up the sample plate prior to transfer to the automated platform.

The Liquidator's flexibility enables quick and easy, one-handed volume change between steps.
It's four working platforms are ideal for moving back and forth between various sample preparation processes.

The Liquidator is then used for adding protein wash buffer during the wash and precipitation stages.
Finally it assists with the sample elution until the plate containing pure DNA is ready for further analyses.

Dr Ivanova says that using the Liquidator 96 is advantageous as it  "..allows you to control the speed for slow aspiration and dispense. You can change volume in a single motion to move to the next step without interruption. This prevents introduction of air bubbles and contamination."

Application 2: DNA Amplification using PCR

To set up PCR plates, 2µl of extracted DNA sample is dispensed into a pre-made PCR plate containing master mix.

Liquidator enables the easy handling of such small volumes.

Application 3: Cycle Sequencing

PCR Reaction Setup
Cycle sequencing analysis is performed to obtain the nucleotide sequence of the Bar Code Region.
It requires highly accurate and precise volume measurements.
Prior to cycle sequencing Liquidator 96 performs 3-fold dilution of the PCR products and then addition to the pre-made sequencing plates.
Product Cleanup
Prior to sequencing the PCR product is cleaned up to remove the dye terminator. Liquidator 96 is used to add the EDTA.

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